Why the Pipette Cannot Suck Properly

There are several reasons for the pipette’s failure to suck properly.

Firstly, we can install the original suction head on the pipette to test the function. If the pipette with the original head can work normally, there is no problem with the pipette. The reason may be the leakage of air caused by the mismatching between the suction head and the pipette.

Secondly, if the pipette is always equipped with the same suction head and cannot suck properly suddenly, the problem is not the suction head. It is very likely that the pipette has not been maintained for a long time. What we can do is remove the suction head thrust ring, suction head conical sleeve, and other parts of the pipette, wipe the piston with alcohol, and remove all kinds of dirt.
And then we can apply the silicone grease to the piston part, re-install the unloaded parts and press the operation button to distribute the silicone grease on the piston to the sleeve. Finishing above, the pipette can be used normally.

Thirdly, if the pipette cannot be used normally after maintenance, the parts of the pipette may be damaged.

In addition, the following questions can also be considered.

Pipette not positioned correctly or used at the wrong angle: The pipette needs to be positioned correctly in the liquid so that the tip is fully immersed in the liquid to ensure effective aspiration. If the angle is not correct, such as tilted or vertical, the liquid may not be picked up properly.

Improper selection of pipette volume: Pipettes have different volume ranges, such as 10 microliters, 100 microliters, etc. If the selected volume is not suitable for the required liquid volume, the exact volume may not be aspirated.

Broken or ill-fitting pipette tips: The quality and condition of the tips is critical for accurate pipetting. If the tip is damaged, deformed or incompatible with the pipette, inaccurate pipetting may result.

Damaged pipette pistons or seals: Damaged, aged, or loose pipette pistons or seals can cause leaks that can affect pipetting accuracy.

Improper technique: Correct pipetting technique is very important for accurate pipetting. If the speed is uneven, the strength is insufficient or too strong during the process of pressing the piston, it may affect the accuracy of suction.

Liquids that are too viscous: Certain liquids have high viscosity, such as thick solutions or liquids that contain high concentrations of solutes. In this case, the pipette may experience pipetting difficulties because the viscosity of the liquid restricts the flow of the liquid.

A pipette may experience issues with proper aspiration for several reasons. Firstly, check for damaged or worn components such as seals, O-rings, and pistons, as they can compromise the pipette’s airtight seal. Contamination in the pipette tip or internal components can impede suction, emphasizing the need for regular cleaning and maintenance. Verify the pipette’s calibration to ensure it is set to the correct volume, preventing inaccuracies in aspiration. Additionally, assess the pipette tip for the correct size and secure attachment.

Improper technique, including incorrect pipetting angles or insufficient immersion depth of the pipette tip, can also hinder proper suction. Electronic or motorized pipettes may encounter issues related to depleted batteries or malfunctioning electronic components.

In summary, addressing issues related to seals, contamination, calibration, technique, and electronic components is crucial to restoring a pipette’s proper aspiration functionality. Regular maintenance and adherence to proper pipetting practices are essential for reliable and accurate pipetting performance.

If the pipette is not pipetting accurately, you can try to check and solve the above problems, such as ensuring the correct angle of placement, replacing the appropriate tip, checking the condition of the pipette’s piston and seal, and ensuring that the correct operating technique is used. If the problem persists, it may be necessary to consult Hawach contact technical support for further help and advice.