Pipette Common Problem Solving Method

The equipment and equipment in the laboratory are as comprehensive as the natural world. They say that the sparrows are small and complete, but in the laboratory, the same small but indispensable instrument has a micropipette for biochemistry. An essential tool for experimental or molecular biology experiments.

The pipette is a precision liquid-removing device that can be adjusted freely within a certain capacity range. It is commonly called a pipetting gun, but there are some common problems such as dripping, leaking, and inaccurate during use.

There are many possible reasons for “dropping”, usually due to the removal of special liquids. Some liquids with high viscosity, high density, and high saturated vapor pressure are often the “culprits” of dripping. When removing such liquids, we recommend a reverse pipetting method.

The main reason for “leakage” is that the pipette is poorly sealed and the tips are not matched. If the experiment repeatedly leaks, the leakiness of the pipette itself is eliminated, and the matching of the tips needs to be considered. Usually the same brand of pipettes and tips are matched to achieve a good system seal.

The main reason for “unacceptable” is that the pipette is not kept vertical when aspirating, or the absorbed liquid is too thick and too volatile. Your pipette may need to be calibrated when you feel that the aspiration is not accurate during the experiment. ISO 9001 and GLP require periodic pipette calibration (approximately every 3-12 months), and pipetting products should be calibrated using the gravimetric method in accordance with ISO 8655. In daily use, it is also necessary to keep the pipette vertical when aspirating. When the liquid is discharged, the tip of the gun is attached to the wall of the container and the angle is maintained at 30-45° with the wall of the container. The pipetting volume is the most accurate.

Hawach can avoid the three major issues of appeal. It combines operational and ergonomic design with advanced and innovative materials and is the perfect manual pipette for scientists who prefer to use the central pipetting button to meet the high operational requirements of the laboratory. All the features required by life science users: Robust construction, one-handed operation, complete sterilization, high precision, and easy calibration technology for long-term reliability.