Introduction of Chemistry, Biology, and Medical Pipette

Pipettes are precision instruments used in laboratories for the accurate measurement and transfer of liquid volumes. Here’s an introduction to chemistry, biology, and medical pipettes, along with their typical applications in each field:

Chemistry Pipettes:

  1. Volumetric Pipettes:
    • Description: Volumetric pipettes are used for measuring a specific volume with high accuracy.
    • Applications: Commonly used in analytical chemistry for precise preparation of standard solutions.
  2. Mohr Pipettes:
    • Description: Mohr pipettes are graduated pipettes that deliver variable volumes up to a maximum volume.
    • Applications: Suitable for general chemistry tasks where high precision is not critical.
  3. Serological Pipettes:
    • Description: Serological pipettes are used for measuring and transferring volumes in biotechnology and molecular biology.
    • Applications: Widely used in cell culture and molecular biology experiments, such as transferring media or reagents.

Biology Pipettes:

  1. Micropipettes:
    • Description: Micropipettes are precision instruments designed for volumes in the microliter range.
    • Applications: Commonly used in molecular biology, genetics, and microbiology for tasks like PCR setup, DNA/RNA quantification, and bacterial culture.
  2. Pasteur Pipettes:
    • Description: Pasteur pipettes are thin, elongated pipettes used for transferring small volumes.
    • Applications: Widely used in microbiology for transferring small amounts of liquids, such as inoculating bacterial cultures.
  3. Transfer Pipettes:
    • Description: Transfer pipettes are disposable plastic pipettes with a bulb at one end.
    • Applications: Used in cell culture and molecular biology for transferring liquids between containers.

Medical Pipettes:

  1. Automatic Pipettes:
    • Description: Automatic pipettes, also known as electronic or motorized pipettes, allow for precise and automated liquid handling.
    • Applications: Commonly used in medical laboratories for tasks like blood sample analysis.
  2. Pipettors for Clinical Chemistry:
    • Description: Specialized pipettors designed for clinical chemistry applications.
    • Applications: Used for handling clinical samples, such as serum or plasma, in diagnostic testing.
  3. Blood Pipettes:
    • Description: Pipettes designed specifically for handling blood samples.
    • Applications: Used in clinical laboratories for accurate measurement and transfer of blood components.

Common Features and Considerations:

  1. Calibration and Accuracy:
    • All types of pipettes must be regularly calibrated to ensure accuracy in volume measurements.
  2. Material:
    • Pipettes are typically made of glass or plastic. Disposable plastic pipettes are commonly used in biology and medical applications to prevent cross-contamination.
  3. Volume Ranges:
    • Pipettes come in various volume ranges, from microliters to milliliters, depending on the application.
  4. Usage Techniques:
    • Proper pipetting techniques, including maintaining the pipette in a vertical position, using the correct tip, and releasing the liquid slowly, are crucial for accurate results.

Understanding the different types of pipettes and their applications is essential for performing precise and reproducible laboratory work in chemistry, biology, and medical settings.

As a useful instrument used to transport a measured volume of liquid, pipettes are widely used in chemistry, molecular biology research, and medical tests too. From electronic pipettes to mechanical pipettes (Standard Single Channel Fixed Pipettes and Advanced Half Autoclave Single Channel Adjustable Pipettes.), you can find Hawach pipettes available in designs for various purposes with different levels of accuracy and precision.

The principle of the pipette is to create a vacuum above the liquid-holding chamber and release some vacuum to draw up and dispense liquid. The original pipette is made of glass which is used with aqueous solutions in the chemistry field. And the piston-driven air displacement pipettes are recommended as the most accurate and precise pipettes.
Standard 8 & 12 Channel Adjustable Pipettes
When you draw up liquid, you’d better dip the tip 3 to 5 mm below the liquid surface at a 90-degree angle. When you dispense, the best way is to hold the pipette at an angle of 45 degrees and place the tip against the side of the receiving vessel. If you have liquid in the tip, remember not to wipe off or blot the tip in any way, even from the exterior, as it will tend to attract and bleed off some of the liquid, with a poor result following.

1. In terms of specifications: because the pipette has only a few fixed specifications, its use is limited. The pipette can be measured at will, so it is widely used. It also depends on the accuracy required by your experiment. Generally, you can choose a suitable pipette for analytical experiments.

2. On the scale: the pipette has a full scale, and the pipette only has a full scale. Pipettes are prone to errors during operation. The pipette is a one-time operation with fewer errors.

In terms of accuracy: a pipette with a scale is called a pipette, a glass tube is called a pipette, or a single standard pipette. The pipette is more accurate than a pipette. Especially when transferring a 20 or 25 ml volume of solution, it is necessary to take a graduated pipette several times to get a large error! Get it all in one go with a pipette!

3. Measure out and measure in: pipettes are used to accurately measure a certain volume of solution. A pipette is a meter-out instrument used only to measure the volume of the solution it emits. The pipette is a straight glass tube with a scale to know the volume of the liquid being measured. According to JJG196-2006, the type mark on the pipette: measuring type: In; measuring type: Ex; blowing type: Blow out.

A pipette is a precise pipette, which is more complicated to use; a pipette is a coarse pipette, which is simpler to use. So labs need to use them in different situations.

The use of pipettes is limited because they are available in a few fixed sizes. The pipette can be measured at will, so it is widely used. It also depends on the accuracy required by your experiment. Generally, you can choose a suitable pipette for analytical experiments.