Pipette Suction Angle

When you use the pipette ( such as Advanced Half Autoclave Single Channel Adjustable Pipettes, Advanced Half Autoclave Single Channel Fixed Pipettes, Advanced Fully Autoclave 8/12 Channels Adjustable Pipettes), the pipette suction angle refers to the angle formed between the axis of the pipette and the vertical axis when a liquid is aspirated into the pipette. This angle is crucial in pipetting techniques, especially in achieving accurate and precise volume measurements. The proper suction angle helps minimize errors in pipetting and ensures reproducibility in laboratory work.

Here are some key points related to the pipette suction angle:

Importance of Pipette Suction Angle:

  1. Accuracy:
    • The suction angle influences the accuracy of the volume measurement during pipetting. An incorrect angle may lead to imprecise liquid transfer.
  2. Precision:
    • Maintaining a consistent suction angle from one pipetting operation to another enhances precision in volume delivery.
  3. Meniscus Formation:
    • The angle affects the shape and position of the liquid meniscus inside the pipette tip. A proper meniscus ensures accurate volume readings.

Best Practices:

  1. Vertical Descent:
    • When aspirating a liquid into the pipette, it is recommended to keep the pipette in a vertical descent position. This helps in forming a uniform meniscus.
  2. Consistency:
    • Maintain a consistent suction angle for reproducibility. This is especially important when pipetting multiple samples or performing serial dilutions.
  3. Avoid Air Bubbles:
    • A proper suction angle helps minimize the introduction of air bubbles during aspiration, which can affect volume accuracy.
  4. Eyeball Level:
    • Keep the eye level parallel to the meniscus to achieve accurate volume readings. This is particularly important when using graduated pipettes.

Common Pipetting Techniques:

  1. Dispensing Technique:
    • When dispensing, it is recommended to touch the pipette tip to the inner wall of the receiving vessel. This ensures complete transfer and minimizes liquid retention.
  2. Slow Aspiration:
    • Aspirate liquids slowly to avoid turbulence and ensure accurate volume measurement.
  3. Release Technique:
    • Release the liquid smoothly without any sudden jerks or movements to prevent splashing and ensure precise delivery.

Instrument-Specific Considerations:

  1. Adjustable Pipettes:
    • Some pipettes allow the adjustment of the angle for ergonomic purposes. Users should follow the manufacturer’s guidelines when making adjustments.
  2. Electronic Pipettes:
    • Electronic or motorized pipettes may have specific guidelines regarding the angle of use. Users should refer to the instrument’s manual for proper handling.
Standard Single-Channel Fixed Pipettes
Advanced Half Autoclave 8 & 12 Channel Adjustable Pipettes
Electronic Control Digital Pipette Epipette

Maintaining a proper pipette suction angle is part of a good pipetting technique, contributing to the overall accuracy and precision of laboratory measurements. Following best practices and being consistent in pipetting procedures are essential for reliable results in various scientific applications.

To facilitate the observation of the condition of the sample in the tip during the pipetting process, many users will keep the pipette at an angle of 45 degrees or even closer to the horizontal angle during the pipetting process. But this is wrong again.

1. When removing a sample that approaches or reaches the maximum volume of the tip, the large angle of the pipette can easily cause the liquid to slip into the handle of the pipette, thereby contaminating or even corroding the piston of the pipette. Causing cross-contamination of the sample.

2. The large angle of the pipette tilt will also increase the contact surface of the outer wall of the tip with the liquid so that the liquid remaining on the outer wall when the tip leaves the liquid surface is.

It is easy to follow the inside of the tip when draining The liquid leaves the tip together, which reduces the precision of the pipetting, and for these small amounts of liquid (for example, 2 ul and 10 ul) pipettes, the damage to the accuracy is likely to be fatal!