How to Keep the Accuracy of the Pipette

Maintaining the accuracy of a pipette is crucial for obtaining reliable and precise results in laboratory work. Here are some key practices to ensure the accuracy of your pipette:

  1. Calibration and Verification:
    • Regularly calibrate your pipettes using calibrated weights or a calibration system. Additionally, verify the accuracy using a quality control solution. Follow the manufacturer’s recommendations for calibration intervals.
  2. Use Proper Technique:
    • Adhere to proper pipetting technique. This includes holding the pipette vertically, immersing the tip to the correct depth, and dispensing at a consistent speed. Inaccuracies can occur if the technique is inconsistent.
  3. Pre-Wetting the Pipette Tip:
    • Pre-wet the pipette tip with the liquid you are pipetting. This ensures that the entire volume is delivered to the sample and reduces the chances of meniscus adhesion.
  4. Selecting the Correct Pipette Tip:
    • Use pipette tips that are recommended by the pipette manufacturer. Using the wrong type or size of tip can affect accuracy.
  5. Avoid Air Bubbles:
    • Ensure there are no air bubbles in the pipette tip or liquid. Air bubbles can lead to inaccurate volume measurements.
  6. Maintain Consistent Temperature:
    • Keep the pipettes and solutions at a consistent temperature. Temperature variations can affect the density of the liquid and, consequently, the volume dispensed.
  7. Regular Cleaning and Maintenance:
    • Clean pipettes regularly to prevent contamination. Follow the manufacturer’s guidelines for cleaning and maintenance. Check for any damage or wear, and replace parts as needed.
  8. Avoid Over-Aspiration and Over-Dispensing:
    • Do not over-aspirate or over-dispense liquids. Ensure that you follow the pipette’s volume range and avoid using the pipette at the extremes of its capacity.
  9. Use the Correct Pipette for the Volume:
    • Select the appropriate pipette for the volume you need to dispense. Using a pipette that is not designed for the desired volume can lead to inaccuracies.
  10. Storage Conditions:
    • Store pipettes in a clean and controlled environment. Protect them from dust, contaminants, and extreme temperatures. Consider using pipette stands or racks for proper storage.
  11. Regular Performance Checks:
    • Implement a routine schedule for checking the performance of your pipettes. This can include gravimetric testing, where the dispensed volume is weighed, or using a calibration system.
  12. Training and Documentation:
    • Train laboratory personnel on proper pipetting techniques and the importance of accurate pipetting. Maintain documentation of calibration, maintenance, and any issues encountered.

By incorporating these practices into your pipetting routine, you can contribute to the accuracy and precision of your laboratory measurements. Regular calibration, proper technique, and attention to detail are key elements in maintaining the reliability of pipettes. The pipette is a kind of accurate instrument for quantitative transferring and weighing liquid in biology and chemistry laboratories.

The most relative element to keep the pipette accurate is tightness. There are several main factors affecting the tightness of the pipette.

The first factor is the quality of the piston. The material of the piston is important and affects the quality of the piston. The piston can withstand the long-term friction with the sleeve.

The second factor is the quality of the sleeve. It should be not easy to bend, expand or shrink.

The third factor is the O-type elastic sealing ring. The O-type elastic sealing ring is consumables. You must check and replace it periodically, or the volume of the suction liquid will be wrong because of the leakage.

The fourth factor is the vacuum grease. The pipette needs to be repaired with grease after cleaning once there is some liquid entering into the pipette accidentally. Generally speaking, the vacuum grease is usually repaired by the manufacturer during calibration.