Six Steps Used By The Pipette
In the research of analysis and testing, the pipette is usually used to take a small amount or a small amount of liquid. For the proper use of the pipette and some details of its operation, many people will ignore, several aspects described in detail.
Hawach summarizes the six steps used by pipettes when you use single-channel pipettes and multi-channel pipettes: such as Standard Single Channel Adjustable Pipettes, Standard Single Channel Fixed Pipettes, Advanced Half Autoclave 8/12 Channels Adjustable Pipettes.
1. Filling the tip
The pipette sleeve is pressed down firmly and can be rotated a small amount when needed.
Wrong operation: Hit the tip with force. This method can cause damage to the pipette tip or even wear of the pipette sleeve, which affects its sealing.
2. Range setting
Please select the correct pipette before the operation. The pipette can operate within the range of 10-100%, but the operation at the 10% range requires high pipetting skills, so it is recommended to operate within the range of 35-100%.
Range adjustment: When adjusting from a small range to a large range, it will rotate continuously in the direction of the required range, and the rotation will reach more than 1/3 turn of the required range and then call back to the required range. When adjusted from a large range to a small range, it is directly rotated continuously to the required range.
When adjusting the range, if you want to adjust from a large volume to a small volume, according to the normal adjustment method, clockwise rotation of the knob can be done. However, if you want to adjust from a small volume to a large volume, you can rotate the scale knob counter-clockwise to the scale beyond the range, and then back to the set volume, so as to ensure the highest accuracy of the measurement.
In this process, do not screw the button out of the range, otherwise, it will jam the internal mechanical device and damage the pipette tip.
Repeat the aspirate and drain two to three times with the same sample, and rinse to provide the same contact surface for each aspiration to ensure the consistency of operation.
Note: Please do not rinse the high-temperature or low-temperature liquid!
4. Tip immersion angle
Try to keep the pipette vertical when aspirating, and the angle of inclination should not exceed 20°.
5. Tip immersion depth
6. Tip immersion time
After aspiration, hold the liquid in the liquid for one second and then gently remove the tip, which is especially important for large-capacity pipettes or for viscous samples.
7. Suction speed
Continuously and continuously pipetting, control the pipetting speed, too fast will cause the spray, liquid, or aerosol to rush into the pipette, contaminating the piston and other components.
8. Draining and blowing
First, press the piston to the first gear to discharge the liquid. After a pause, press the second gear to blow the liquid.
9. Hand temperature
The pipette should not be held in your hand for a long time. It is best to hang it on the stand or hang it on your hand when not in use.
After use, you can hang it vertically on the pipette stand, but be careful not to fall off. When there is liquid in the piston tip of the pipette, do not put the pipette horizontally or inverted, so as to avoid the liquid backflow corroding the piston spring.