Use Precautions Of Pipette
It is very crucial to correctly and properly use the pipette, which is worthy of being paid great attention to from the operators. To prevent the pipette damage and personal injury, to learn about the use of precautions is very necessary.
First of all, remember do not pipette if the tip is not installed. At the beginning of the sample pipetting operation, it is necessary to keep it slow and stable by preventing the sample from entering the solution extractor, in case of the occurrence of air leakage due to the excessive speed. To obtain high precision, it is essential to extract the sample solution once before the formal pipetting, especially when dealing with the serum protein solution or organic solvent.
It is possible to form a “liquid film” on the inner wall, which would cause a small drainage volume. In particular, it is strictly forbidden to extract solutions with strong volatility and strong corrosiveness, such as concentrated acid and alkaline.
Check for leakage during the use of a pipette. The method is to extract the solution and put it vertically for a few seconds to observe whether the solution level has dropped. Also, make sure the pipette can adapt to high temperatures before being autoclaved.