Matters Needing Attention When Using Hawach Pipette

The correct placement of the Hawach pipette-use it, you can hang it vertically on the pipette holder, but be careful not to fall off. When there is liquid on the top of the pipette, do not place the pipette horizontally or upside down to avoid liquid reflux corrosion of the piston spring.

When using Hawach pipettes or any other precision laboratory instruments, it’s crucial to follow proper techniques and precautions to ensure accurate and reliable results. Here are some important considerations:

  1. Calibration and Verification:
    • Ensure that the pipette is properly calibrated and verified according to the manufacturer’s instructions before use. Regular calibration is essential for accuracy.
  2. Proper Handling:
    • Handle the pipette with care. Avoid dropping or mishandling it to prevent damage to the instrument.
  3. Selecting the Right Pipette:
    • Choose the appropriate pipette for the desired volume range. Using the correct pipette ensures accurate and precise dispensing.
  4. Tips for Aspiration and Dispensing:
    • Use smooth, controlled motions during aspiration and dispensing to prevent sample loss or inaccuracies.
  5. Avoid Pipetting by Mouth:
    • Never pipette by mouth. Use a proper pipette aid or electronic pipette for aspiration and dispensing.
  6. Avoid Contamination:
    • Use a new, clean tip for each aspiration to prevent cross-contamination between samples.
  7. Correct Tip Attachment:
    • Ensure that the pipette tip is securely attached to the pipette nozzle to prevent leaks or loss of sample.
  8. Sample Consistency:
    • Ensure that the sample is uniform in consistency and free from any particulates or air bubbles that may affect the accuracy of pipetting.
  9. Avoid Over-Pipetting or Under-Pipetting:
    • Follow the recommended pipetting technique to avoid over-dispensing or under-dispensing of the sample.
  10. Pre-Wetting Pipette Tips:
    • Pre-wet the pipette tips with a small volume of the sample or buffer solution before aspirating to ensure accurate volume delivery.
  11. Avoid Plunging the Pipette Too Deeply:
    • When aspirating, avoid plunging the pipette too deeply into the solution to prevent contact with the sample and possible contamination.
  12. Temperature Considerations:
    • Be aware of temperature effects on sample volumes. Adjust for temperature differences according to the manufacturer’s recommendations.
  13. Proper Storage:
    • Store the pipette in a clean, designated area, protected from dust and contaminants. Follow manufacturer guidelines for long-term storage.
  14. Regular Maintenance and Cleaning:
    • Clean and maintain the pipette regularly according to the manufacturer’s recommendations to ensure optimal performance.
  15. Documentation:
    • Record the pipette model, calibration date, and any specific details about its use, especially when working with sensitive or critical samples.
  16. Training and Familiarity:
    • Ensure that users are properly trained on the specific pipette model and are familiar with its operation and limitations.

By adhering to these guidelines, you can maximize the accuracy, precision, and longevity of your Hawach pipettes, ultimately ensuring reliable and reproducible results in your laboratory work.

Hawach pipette maintenance:

1. If not in use, adjust the pipette range to the maximum, leaving the spring in a relaxed state to protect the spring
2. Cleaning the pipette regularly. It can be washed with soapy water or 60% isopropanol, distilled water, and dried naturally.
3. Before high-temperature sterilization, make sure the pipette can adapt to high temperatures.
4. Calibration allows repeated weighing of distilled water at 20 to 25 degrees Celsius.5. Check if there is any leakage during use. The method is to take the liquid and then hang it vertically for a few seconds to see if the liquid level drops. If there is leakage, the reasons are as follows:
(1) Whether the pipette heads match;
(2) Whether the spring piston is normal;
(3) If it is a volatile liquid (like many organic solvents), it may be a matter of vapor pressure. You can suck the liquid several times through a pipette.