Drain and Suction of A Pipette

A pipette is an indispensable tool for experimental operation. The most important thing is that the experiment has a minimum error and good repeatability.

In the process of using a pipette, please note:

1. Low environment temperature and high hand temperature makes the air expand. Error often occurs when the cold solution is inhaled.
2. The joint part of the tip and body has been worn away for a long time, resulting in errors.
3. It is easy to cause bubbles in the tip and pollute the tip.
4. When clean a pipette, the same sample is used for repeated suction and drainage for three times.
5. The steam of the reagent with high volatility will enter the tip, and the internal pressure will increase. As a result, the pressure will increase when the liquid is pressed out, resulting in errors.

Draining a Pipette:

  1. Insert Pipette Tip:
    • Attach a clean and appropriately sized pipette tip to the pipette.
  2. Submerge Pipette Tip:
    • Submerge the tip of the pipette into the liquid sample. Make sure the tip is fully immersed.
  3. Depress Plunger Slowly:
    • Depress the plunger slowly and steadily. This creates a vacuum within the pipette, allowing the liquid to be drawn up into the tip.
  4. Wait for Equilibration:
    • Allow the liquid to equilibrate in the pipette tip for a moment to ensure an accurate measurement.
  5. Release Plunger:
    • Slowly release the plunger to expel any excess liquid back into the original container.
  6. Remove Pipette Tip:
    • Carefully remove the pipette tip from the sample.

Suctioning a Pipette:

  1. Attach Pipette Tip:
    • Attach a clean and appropriately sized pipette tip to the pipette.
  2. Adjust Volume Setting:
    • Set the pipette to the desired volume using the adjustment dial or knob. Ensure the volume setting matches the intended volume to be aspirated.
  3. Submerge Pipette Tip:
    • Submerge the tip of the pipette into the liquid to be aspirated. Ensure the tip is fully immersed.
  4. Depress Plunger Fully:
    • Depress the plunger fully and swiftly. This creates a vacuum in the pipette, drawing the liquid into the tip.
  5. Wait for Equilibration:
    • Allow the liquid to equilibrate in the pipette tip for a moment to ensure an accurate measurement.
  6. Withdraw Pipette Tip:
    • Carefully remove the pipette tip from the liquid source.
  7. Dispense Liquid:
    • Hold the pipette over the target container and slowly release the plunger to dispense the liquid.
  8. Expel Any Residual Liquid:
    • After dispensing, expel any residual liquid in the pipette tip by depressing the plunger to the first stop.

Remember to always handle pipettes with care to prevent damage or contamination. Additionally, calibrate and maintain pipettes regularly to ensure accurate and reliable measurements. A pipette should not be held in the hand for a long time, it is best to hang it on support when not in use.