How to Choose the Right Range of Pipettes?

Hawach often receives such inquiries from customers, “Help me recommend pipettes, which can be used in all ranges, four sets.” At the request of the customer, we will generally recommend 0.5 to 10uL, 5-50uL, 10-100uL, 100-1000uL pipettes for the four-range range. On the surface, the customer bought a full scale that covered almost all the micropipetting. It seems that everything is perfect, but it is not the case! After many years of pipette after-sales work, feedback on customer issues received, such as inaccurate pipetting, not easy to use, is often derived from the initial suit selection.

Let’s take one of the most common examples: the most common feedback from customers in pipetting is to use a 10uL pipettes to transfer liquids up to 1uL. Theoretically, a pipette with a range of 0.5-10 uL is not problematic when operating in a liquid within 0.5-1 uL. Why is it that during this range, customers often have the most feedback. Below 1uL is an ultra-micro liquid operation. The amount of liquid is almost invisible to the naked eye. The basic work requirements for liquid handling are very high, such as washing, inserting liquid depth and other technical details. In addition, the quality of the tip also has a great influence on this micro-operation. Some tips with poor quality have residual plastic burrs at the tip end of the tip. This poor quality tip has a fatal effect on micropipetting. In the case of using a normal-quality tip, the mass operator chooses to use pipettes of 0.1-2uL range, and the liquid transfer operation below 1uL is significantly better than the 0.5-10uL range pipette.

Choosing the right range of pipettes is crucial for accurate and precise liquid handling in the laboratory. Here are steps to help you select the appropriate pipette range:

1. Determine the Volume Range Needed:

• Consider the volume range of your samples. Make sure to choose pipettes that can handle the lowest and highest volumes you commonly work with.

2. Identify the Specific Volumes Used:

• Take note of the specific volumes you most frequently handle. This will help you prioritize the selection of pipettes within those ranges.

• Look at the gradations or increments on the pipettes. Ensure they match the level of precision required for your experiments.

4. Evaluate the Accuracy and Precision Requirements:

• Different experiments may have varying requirements for accuracy and precision. Select pipettes with specifications that meet these criteria.

5. Determine the Number of Pipettes Needed:

• Decide whether you need a single-channel pipette or if a multi-channel pipette (which can handle multiple samples simultaneously) would be more efficient for your work.

6. Consider Specialty Pipettes:

• Depending on your specific applications, you may require specialty pipettes like adjustable volume or positive displacement pipettes. Evaluate if these are necessary for your work.

• Consider the ergonomic design of the pipettes. Choose pipettes that are comfortable to hold and operate, especially for tasks that require extensive pipetting.

8. Verify Calibration and Maintenance:

• Ensure that the pipettes you choose can be easily calibrated and maintained for accuracy and reliability.

9. Take Budget into Account:

• Consider your budget constraints. While high-end pipettes offer advanced features, there are also reliable mid-range options available.

10. Choose a Reputable Brand:

• Opt for well-known brands with a reputation for producing high-quality and reliable pipettes. This can ensure that you are investing in a reliable tool.

11. Consider Future Needs:

• Think about the potential expansion of your work. If you anticipate working with a wider range of volumes in the future, consider pipettes that can accommodate those needs.

12. Seek Input from Colleagues or Experts:

• If possible, consult with experienced colleagues or experts in your field for advice on selecting the most suitable pipettes for your specific applications.

By carefully considering these factors, you can choose a range of pipettes that will best suit your laboratory’s needs and ensure accurate and precise liquid handling in your experiments.

Hawach provided Advanced Fully Autoclave 8/12 Channels Adjustable Pipettes, Advanced Half Autoclave Single Channel Fixed Pipettes, Standard Single Channel Fixed Pipettes, and other pipettes for your choose.