How to Choose Suitable Pipette Tips?

Tips, as consumables used with pipettes, generally can be divided into standard tips; filtered tips; low adsorption tips and conductive filter pipette tips, etc.

1. The standard tip is a widely used tip. Almost all pipetting operations can use ordinary tips, which are the most affordable type of tips.
2. The filtered tip is a consumable designed to avoid cross-contamination and is often used in experiments such as molecular biology, cytology, and virology.
3. The surface of the low-adsorption tip has undergone a hydrophobic treatment, which can reduce the low surface tension liquid leaving more residues in the tip.
PS: The wide-mouth tip is ideal for sucking viscous materials, genomic DNA, and cell culture fluid.

How to choose a good tip?

The statement can be said to be partly true but not entirely true. The tip that can be mounted on the pipette can indeed form a pipetting system with the pipette to realize the pipetting function, but is this reliable? A question mark is required here.

10ul-sterile-filter-pipette tips 300ul conductive tips

Choosing suitable pipette tips is crucial for accurate and precise liquid handling in the laboratory. Several factors need to be considered to ensure compatibility with your pipette and the specific requirements of your applications. Here are key considerations when selecting pipette tips:

(1). Pipette Compatibility:

  1. Brand and Model:
    • Choose pipette tips that are designed to fit the specific brand and model of your pipette. Different manufacturers may have unique tip designs that ensure proper sealing and accuracy.
  2. Universal Tips:
    • Some tips are designed to be compatible with multiple pipette brands. However, it’s essential to verify compatibility and performance with your specific pipette model.

(2). Volume Range:

  1. Appropriate Volume Range:
    • Select pipette tips that cover the volume range you frequently work with. Tips are often available in various sizes, including low-volume and high-volume options.
  2. Accuracy and Precision:
    • Ensure that the pipette tips contribute to the accuracy and precision of your liquid handling, especially when working with small volumes.

(3). Material:

  1. Polypropylene (PP):
    • Most common material for pipette tips. Offers chemical resistance and is suitable for general laboratory use.
  2. Low Retention Tips:
    • Some applications may benefit from low retention tips designed to minimize sample residue left on the tip.

(4). Sterility:

  1. Sterile Tips:
    • Choose sterile tips for applications where contamination is a concern, such as cell culture or PCR.

(5). Filter Tips:

  1. Aerosol Barrier Tips:
    • Include filters to prevent aerosol contamination during pipetting. Suitable for sensitive applications like PCR.

(6). Racked or Reload Tips:

  1. Racked Tips:
    • Preloaded tips in racks for convenience and to minimize contamination during handling.
  2. Reload Tips:
    • Economical option where tips are manually loaded into empty racks.

(7). Specialty Tips:

  1. Conductive Tips:
    • For applications where electrostatic discharge needs to be minimized, such as working with sensitive electronic components.
  2. Wide Orifice Tips:
    • Suitable for viscous samples or samples containing particulate matter.

(8). Tip Design:

  1. Filtered or Non-Filtered:
    • Choose between filtered and non-filtered tips based on the nature of your samples and applications.
  2. Extended Length Tips:
    • Some applications, such as reaching the bottom of deep tubes or wells, may benefit from extended length tips.

(9). Color Coding:

  1. Color-Coded Tips:
    • Some brands use color-coding for quick identification of tip sizes or types.

(10). Quality and Certification:

  1. Quality Assurance:
    • Choose tips from reputable manufacturers with quality control measures and certifications.
  2. RNase/DNase-Free and Non-Pyrogenic:
    • Especially important for molecular biology and cell culture applications.

Additional Tips:

  • Tip Ejection System:
    • Ensure that the pipette tips are compatible with the ejection system of your pipette.
  • Environmental Considerations:
    • Some laboratories prioritize using eco-friendly or low-waste options for pipette tips.
  • Bulk Packaging:
    • For high-throughput labs, consider bulk packaging options to reduce packaging waste.

Always refer to the manufacturer’s guidelines and recommendations for pipette tips to ensure optimal performance and reliability. Regularly inspect and maintain your pipette tips to prevent issues related to contamination or inaccurate pipetting.

Tip features of the pipette tip

So what are the minimum points that a good tip must-have?
A good tip depends on concentricity, taper, and the most important point is adsorption;
1. Let’s talk about the taper first: if it is better, the match with the pipette is very good.
2. Concentricity: Concentricity is whether the circle between the tip of the tip and the link between the tip and the pipette is the same center. If it is not the same center, it means that the concentricity is not good;
3. Finally, the most important one is our absorptivity: absorptivity is related to the material of the tip. If the material of the tip is not good, it will affect the accuracy of pipetting and cause a large amount of liquid to be retained or referred to as hanging on the wall, causing errors in pipetting.

So everyone should pay special attention to the above three points when choosing a pipette tip. A row of bad tips is clearly spaced differently! You will see obvious distortions, but this is a very important step in choosing a good tip. Also, please note the installation of the tips on the single-channel pipette and the multi-channel pipette is different. For the single-channel one, insert the tip vertically into the pipette tip, press lightly, and turn it slightly to tighten it. For multi-channel, the multiple channels of the pipette should be aligned with multiple tips, inserted at an angle, and slightly shake back and forth to tighten; Do not repeatedly hit the pipette to ensure the airtightness of the tip.

Answering this question requires data to speak

1. Match the pipette with the tip for performance testing.
2. Calculate the accuracy of the pipetting operation after converting it into volume according to the density of the test liquid.
3. What we have to choose is to have a good tip. If the pipette and the tip are not well-matched, it means that the tightness of the tip and the pipette cannot be guaranteed, making the results of each operation impossible to reproduce.