In severe cases, the pipette will be damaged, so such operations should be avoided. The pipette does not have an O-ring, and it is matched with a tip with a front stop point. The ideal seal can be achieved with just one light pressure, which is a boon for users of multichannel pipettes. The selection of pipette tips carefully:
Material of Pipette Tips
Low retention tips on the market are basically made of polypropylene plastic. High-quality tips are generally made of natural polypropylene, while cheap tips are likely to use recycled polypropylene plastic. In addition, most of the tips will add a small number of additives during the manufacturing process. The more common additives include color-developing materials and release agents. Try to choose a pipette tip with fewer additives in choosing the process to enhance performance.
Packaging of Pipette Tips
The main packaging forms are bagged and boxed. Bagging is to pack the tip in a plastic ziplock bag. Most users will manually put the tip into the tip box after buying the bagged tip, which will increase the chance of contamination of the tip during this process. In recent years, there has also been a refill (the tip is quickly put into the tip box without touching the tip). This kind of pipette tip requires less storage space, reduces the use of plastic, and achieves the goal of environmental protection.
Liquid Sucking of Pipette Tips
Be sure to loosen your thumb slowly and steadily when sucking liquid, and never loosen it suddenly, so as to prevent the solution from sucking in too fast to contaminate the plunger or cause leakage risk.
Compensation Amount of Pipette Tips
Liquids or samples which are featured in high viscosity will cause potential errors in the operation process. In order to avoid the above errors, the compensation amount of samples should be decided by actual need. The compensation amount can be set by changing the reading window of the adjustment knob. It is better not to mix samples or liquids.