Analyze How the Pipettes are Sterilized and Disinfected
The concept of disinfection and sterilization is often used in the use of molecular tools for molecular biotechnology. The difference between the two is that disinfection only requires that the live bacteria on the pipette be controlled within a certain range to achieve a level of harmlessness, while sterilization requires the elimination of all live bacteria. Sterilization is more demanding than disinfection.
Pipette Disinfection:
1. Chemical disinfection. Simply put, wipe the outer surface of the pipette with alcohol, ethanol, isopropanol, or quaternary ammonium compounds (quats),etc., and then dry it. Pipette surfaces are wiped down with a cloth or swab soaked in the disinfectant. This method is suitable for routine disinfection between uses. This should be achievable for all pipette brands, otherwise, the material of the outer casing is too bad! This method is suitable for routine disinfection between uses.
2. UV disinfection. The surface of the pipette is irradiated with ultraviolet rays to achieve the purpose of disinfection by destroying the DNA structure of the cells. The duration of UV disinfection depends on the intensity of the radiation and the resistance of the bacteria to UV light. Most brands of pipettes are UV sterilizable but need to be confirmed with the supplier beforehand.
3. UV-C Light Disinfection. UV-C light is a germicidal ultraviolet light that can kill or inactivate microorganisms. Some laboratories use UV-C light boxes or chambers designed for pipette disinfection. Pipettes are placed in these chambers, and the UV-C light is used to disinfect the surfaces.
Pipette Sterilization:
The pipette is autoclaved. Generally, the working conditions of pipette autoclaving are 102.9 kPa (1.05 kg/cm2), 121 ° C, 20 minutes. The high temperature and high-pressure sterilization of pipettes are divided into whole sterilization and half sterilization. Most pipettes of most brands can be sterilized in half, but whether it can be sterilized, users must supply and supply. Confirmed by the business.
1. Autoclaving: Autoclaving is a common method used to sterilize pipettes. Pipettes are placed in an autoclave chamber and subjected to high-pressure steam at elevated temperatures (usually around 121°C) for a specified period (usually 15-30 minutes). This process effectively kills or inactivates most microorganisms, including bacteria, spores, and viruses.
2. Dry Heat Sterilization: Some pipettes, particularly those with electronic components that cannot withstand moisture, may be sterilized using dry heat. This involves placing the pipettes in an oven set to a high temperature (typically around 160-180°C) for a specific duration (usually 2-4 hours).
3. Gamma Irradiation:
In the market, products such as Advanced Half Autoclave 8/12 Channels Adjustable Pipettes can be sterilized. But here are a few reminders:
(1) In addition to individual applications, such as infectious virus research, most customers do not need whole sterilization;
(2) For products of the same brand, the whole sterilization is often more than half sterilized, and the customer needs to make a decision according to his own needs.
Gamma irradiation involves exposing the pipettes to ionizing radiation from a gamma source. This method is commonly used for sterilizing pipette tips, but may also be used for certain types of pipettes.
Considerations for Sterilization and Disinfection:
Material Compatibility: Different pipettes are made from various materials, and not all may be suitable for every sterilization or disinfection method. For instance, pipettes with electronic components may not be compatible with autoclaving.
Effect on Calibration: Sterilization and disinfection methods should not compromise the accuracy or calibration of the pipette. Some methods, like autoclaving, may require re-calibration after the process.
Frequency of Sterilization/Disinfection: The frequency of sterilization or disinfection depends on the nature of the work being performed. Pipettes should be sterilized before use, and disinfected between uses if necessary.
User Safety: When using chemical disinfectants, appropriate personal protective equipment (PPE) should be worn to protect against potential skin or respiratory irritation.
Proper Handling: Pipettes should be handled carefully during sterilization or disinfection to avoid damage or contamination.
Validation and Documentation: Laboratories should have protocols in place for validating and documenting the effectiveness of sterilization and disinfection processes to ensure compliance with quality assurance standards.
It’s important to note that the specific methods and protocols for sterilization and disinfection may vary depending on the type of pipette, manufacturer recommendations, and laboratory-specific protocols. Always refer to the manufacturer’s instructions and the laboratory’s standard operating procedures for guidance.
