Analyze How the Pipettes are Sterilized and Disinfected
The concept of disinfection and sterilization is often used in the use of molecular tools for molecular biotechnology. The difference between the two is that disinfection only requires that the live bacteria on the pipette be controlled within a certain range to achieve a level of harmlessness, while sterilization requires the elimination of all live bacteria. Sterilization is more demanding than disinfection.
1. Chemical disinfection. Simply put, wipe the outer surface of the pipette with alcohol, etc., and then dry it. This should be achievable for all pipette brands, otherwise, the material of the outer casing is too bad!
2. UV disinfection. The surface of the pipette is irradiated with ultraviolet rays to achieve the purpose of disinfection by destroying the DNA structure of the cells. The duration of UV disinfection depends on the intensity of the radiation and the resistance of the bacteria to UV light. Most brands of pipettes are UV sterilizable but need to be confirmed with the supplier beforehand.
The pipette is autoclaved. Generally, the working conditions of pipette autoclaving are 102.9 kPa (1.05 kg/cm2), 121 ° C, 20 minutes. The high temperature and high-pressure sterilization of pipettes are divided into whole sterilization and half sterilization. Most pipettes of most brands can be sterilized in half, but whether it can be sterilized, users must supply and supply. Confirmed by the business. In the market, products such as manual pipettes can be sterilized. But here are a few reminders:
(1) In addition to individual applications, such as infectious virus research, most customers do not need whole sterilization;
(2) For products of the same brand, the whole sterilization is often more than half sterilized, and the customer needs to make a decision according to his own needs.