About the Structure and Working Principle of Pipette

The structure and working principle

The pipette generally includes a control match button (different manufacturers have different designs, and this button is usually used to adjust the aspiration volume), tip push button, volume display window, sleeve, elastic nozzle, and tip.

The essence of the pipette is a piston pipette, which uses the principle of air discharge to work, and the capacity of the pipette is determined by the distance moved in the piston sleeve. According to different pipetting modes, it can be divided into built-in piston pipetting mode and external piston pipetting mode. Our daily use is the built-in piston pipetting mode.

Most of the tips with built-in piston pipetting mode are universal, but cross-contamination is more likely to occur during operation, and it is not suitable to suck high-viscosity liquids and volatile liquids; In the external piston pipetting mode, there is no air segment between the piston and the liquid. The piston is disposable. It can also achieve accurate pipetting for liquids with larger viscosity.

In addition, because there is no air gap, the sample is avoided. Due to the cross-contamination of aerosols that may occur in air contact, it is very suitable for the removal of precious reagents and biological samples.

For taking and dispensing of liquids when using the pipette, the pipette tip is disposable attachments, which are used to prevent cross-contamination from one sample to another.

When samples are aspirated into a pipette tip, a fine mist may be created, which can get into the pipette at the same time. And the aerosol can contaminate the next sample very easily even after you change the tip quickly. The fragment carried over from the previous sample might be just a tiny bit for most applications, but it still can cause big problems, especially for sensitive applications.

Sometimes, it is hard to disassemble and clean a pipette after every sample in most labs. To capture the aerosols, what we need is nothing but pipette tips, which can not only protect the pipette from contamination from samples but protect the samples from contamination from the pipette as well.

Made of polypropylene, Hawach pipette tips are clear to allow for an easy view of the contents of the tip. You can find Hawach pipette tips non-sterile and sterile, and both of them are DNase, RNase, DNA, and pyrogen-free.