What Causes the Inconsistency of Multi-channel Pipette Tips?

Pipettes are often used in laboratories, schools, research institutes, hospitals, etc. Among the many pipette types, multi-channel pipettes are pipettes with multiple tips, so some users are in use. It is found that the pipette tip suction amount is inconsistent. What causes the multi-channel pipette tip suction amount to be inconsistent? How to solve it?

  1. Tip Quality:
    • Cause: Poorly manufactured or low-quality tips can have variations in dimensions and materials.
    • Solution: Use high-quality, certified pipette tips from reputable manufacturers.
  2. Tip Fit:
    • Cause: Improper seating of the tips on the pipette channels can lead to variations in sample delivery.
    • Solution: Ensure that tips fit securely on all channels, and use tips specifically designed for the particular multi-channel pipette.
  3. Contamination:
    • Cause: Contaminants on the inside or outside of the tips can affect sample accuracy.
    • Solution: Keep tips clean and avoid touching the inside surfaces. Use filtered tips for applications where sample purity is critical.
  4. Sample Viscosity:
    • Cause: Samples with high viscosity or unusual properties may not flow consistently through all channels.
    • Solution: Choose appropriate tips and pipetting techniques for the viscosity of the sample.
  5. Calibration Issues:
    • Cause: Lack of regular pipette calibration can result in inaccurate volume delivery.
    • Solution: Calibrate the multi-channel pipette regularly according to the manufacturer’s recommendations.
  6. Pipette Maintenance:
    • Cause: Lack of proper maintenance, including cleaning and lubrication, can affect pipette performance.
    • Solution: Follow the manufacturer’s maintenance guidelines, regularly clean and lubricate the pipette, and inspect for any damage.
  7. User Technique:
    • Cause: Inconsistent user techniques, such as variations in pipetting speed or angle, can contribute to inconsistencies.
    • Solution: Train users in proper pipetting techniques, and ensure that they follow recommended procedures consistently.
  8. Temperature and Humidity:
    • Cause: Environmental conditions can impact the consistency of pipetting.
    • Solution: Work in controlled laboratory conditions, and be aware of temperature and humidity variations that might affect pipetting accuracy.
  9. Tip Compatibility:
    • Cause: Using tips from different manufacturers may lead to inconsistencies.
    • Solution: Use tips recommended by the multi-channel pipette manufacturer for optimal compatibility.
  10. Tip Racks:
    • Cause: Poor quality or damaged tip racks can contribute to inconsistencies.
    • Solution: Use undamaged, high-quality tip racks and store them properly.
  11. Air Bubbles:
    • Cause: Trapped air bubbles in the tips can disrupt volume accuracy.
    • Solution: Ensure that tips are filled and dispensed without introducing air into the system. Use proper pipetting techniques to minimize air entrapment.
  12. The piston is polluted:

    • Cause:  Pipettes that use long or irregular operations (such as pipetting the liquid beyond the range of the tip will cause liquid to flow into the pipette) can cause the pipette to accumulate.
    • Solution: After opening, you can use a cotton swab or a soft brush to pick up a small amount of water to clean the stains. For more stubborn substances, rinse the pipette with plenty of water, then dry naturally or in an oven at 60 °C. Do not use organic solvents for cleaning or ultrasonic cleaning.
  13. The tip does not match the pipette:
    • Cause:  The pipette has been used for a long time, and the tip is often replaced. The replacement tip may not be a regular tip or a matching tip, which may easily lead to inconsistent pipette tips.
    • Solution:  Replace the tip, it is recommended to use the original pipette tip.

Hawach provided single pipette and multi-channel pipettes: such as Advanced Fully Autoclave Single-Channel Fixed Pipettes and Advanced Fully Autoclave 8/12 Channels Adjustable Pipettes.