Two Cases When Detecting The Degree Of Pipette
We need to check if the temperature of the pipette is different from the temperature difference between the pipette and the tip, which affects the accuracy of the pipette.
In general, when the temperature of the removed liquid does not match the temperature of the tip and the pipette, the following two differences occur:
1. When the liquid temperature is higher than the pipette tip, the volume of the removed liquid will be too large;
2. When the liquid temperature is lower than the pipette tip, the volume of the removed liquid will be too small.
If the above situation is ruled out, then we will investigate the following situation:
Is there a very significant difference between the density of the liquid sample and the water?
In general, the pipette calibration before leaving the factory is tested with water as a standard solution, so if the specific gravity of the liquid to be treated is very different, the degree and accuracy given may not be met, resulting in The error.
After eliminating all the above problems, we should also pay attention to the liquid absorption speed is not too fast, the liquid absorption speed will produce a backlash and air bubbles too fast, resulting in inaccurate pipetting volume, and ultimately affect the accuracy of the pipette.