Purchase and Maintenance of Pipette

The purchase and maintenance of pipettes are essential aspects of laboratory work to ensure accurate and reproducible liquid handling. Here are guidelines for both acquiring pipettes and maintaining them for optimal performance:

Purchase of Pipettes

1. Assess Laboratory Needs:
Identify the types and volume ranges of pipettes required based on the laboratory’s applications and experiments.

2. Choose Reliable Brands:
Select pipettes from reputable manufacturers known for producing accurate and precise instruments. Consider factors like calibration, ergonomics, and user-friendly features.

3. Consider Calibration and Certification:
Opt for pipettes that come with calibration certificates to ensure accuracy. Some laboratories may require pipettes that comply with international standards and regulations.

4. Adjustable or Fixed Volume:
Depending on the applications, choose between adjustable volume and fixed volume pipettes. Adjustable volume pipettes offer flexibility, while fixed volume pipettes are simpler to use.

5. Ergonomics and User Comfort:
Prioritize pipettes with ergonomic designs and comfortable grips to reduce user fatigue during repetitive pipetting tasks.

6. Compatibility with Tips:
Ensure that the selected pipettes are compatible with a wide range of tips, and consider using filtered tips for applications that require additional protection against contamination.

7. Consider Specialized Pipettes:
Depending on the laboratory’s needs, consider specialized pipettes such as multichannel pipettes, electronic pipettes, or positive displacement pipettes.

8. Supplier Support and Warranty:
Choose suppliers that provide good customer support and warranty services. A reliable warranty ensures that any malfunctioning pipettes can be addressed promptly.

Maintenance of Pipettes:

1. Regular Calibration:
Schedule regular calibration of pipettes to ensure accuracy. Follow the manufacturer’s recommendations for calibration intervals.

2. Use Proper Pipetting Techniques:
Train laboratory personnel on proper pipetting techniques to avoid mechanical stress on pipettes and ensure accurate and precise liquid dispensing.

3. Routine Cleaning:
Clean pipettes regularly to prevent sample carryover. Use mild detergents or recommended cleaning solutions. Pay attention to the pistons, seals, and other critical components.

4. Avoid Contamination:
Minimize the risk of contamination by using disposable tips and ensuring proper handling of pipettes in a clean environment. Pipette tips should be changed for each new sample.

5. Prevent Over-pipetting:
Avoid over-pipetting by using the correct pipette volume setting for the intended application. This helps maintain the accuracy of the pipette.

6. Store Properly:
Store pipettes in an upright position in a designated area. Avoid placing them horizontally or vertically with the tip down to prevent damage and contamination.

7. Handle with Care:
Instruct users to handle pipettes with care. Avoid dropping them, and use a pipette stand when not in use to prevent damage to the tips and other components.

8. Regular Inspection:
Periodically inspect pipettes for signs of wear, damage, or malfunction. Replace any worn or damaged parts promptly.

9. Documentation:
Maintain proper documentation of pipette calibrations, repairs, and any issues encountered. This documentation is crucial for quality control and regulatory compliance.

10. Training Programs:

Implement ongoing training programs for laboratory personnel to ensure that everyone is aware of proper pipetting techniques and the importance of pipette maintenance.

11. Scheduled Service:

Consider scheduling regular servicing by the manufacturer or an authorized service provider to address any technical issues and ensure optimal performance.

12. Quality Pipette Tips:

Use high-quality pipette tips that are compatible with the pipettes. Low-quality tips can lead to inaccuracies and compromise the performance of the pipette.

13. Environmental Conditions:- Store and operate pipettes within the specified environmental conditions outlined by the manufacturer, including temperature and humidity requirements.

14. Retire Old Pipettes:

Retire pipettes that are beyond their recommended lifespan. The frequency of replacement depends on the manufacturer’s recommendations and the level of usage.

By carefully selecting, calibrating, and maintaining pipettes, laboratories can ensure the accuracy and reliability of liquid handling, contributing to the overall quality of experimental results. Following best practices and manufacturer guidelines is key to maximizing the lifespan and performance of pipettes in the laboratory setting.