One is forward pipetting. Press the button with your thumb to the first stop, and then slowly release the button back to the origin(aspirate a fixed volume of liquid). Then press the button to the first stop point to discharge the liquid, and then press the button to the second stop point to blow out the residual liquid. Finally, release the button.
The other is reverse pipetting. This method is generally used to transfer high viscosity liquid, biological active liquid, easily foaming liquid or a very small amount of liquid.
The principle is to inhale more than the set range of liquid first, and do not need to blow out the residual liquid when transferring the liquid, there is no need to blow out the residual liquid. First, press the button to the second stop point, and slowly release the button to the origin point, after sucking up, lean against the container wall to flow excess liquid back to the container along the container wall. Then press the button to the first stop point to discharge the liquid with the set range, continue to hold the button at this (do not press it down again), remove the tip of the residual liquid, discard it and suck it up. Leaning against the container wall to flow excess liquid back to the container along the container wall.
4. Correct placement of pipette
After use, it can be hung vertically on the pipette rack, but be careful not to fall down. When there is liquid in the tip of the pipette, do not place the pipette horizontally or upside down, so as to avoid the backflow of liquid corroding the piston spring.