Pipetting Method of Pipette

Pipetting is a fundamental laboratory technique used for accurately measuring and transferring liquid volumes. There are two main types of pipettes commonly used in laboratories: manual pipettes ( Advanced Half Autoclave 8/12 Channels Adjustable Pipettes, Advanced Fully Autoclave 8/12 Channels Adjustable Pipettes, Standard 8/12 Channels Adjustable Pipettes, Standard Single Channel Fixed Pipettes) and electronic pipettes ( Electronic Control Digital Pipettes). The pipetting method may vary slightly depending on the type of pipette being used.

Manual Pipettes:

1. Setting the Volume:

  • Choose the appropriate volume setting on the pipette by turning the volume adjustment dial or plunger. Make sure it corresponds to the desired volume you need to pipette.

2. Loading the Pipette:

  • Attach a disposable pipette tip to the pipette.
  • Immerse the tip into the liquid sample.
  • Depress the plunger to the first stop (pre-wetting position) and then release it to draw the liquid into the pipette tip.

3. Dispensing the Liquid:

  • Position the pipette tip over the target container.
  • Depress the plunger to the first stop to expel the majority of the liquid.
  • To ensure complete dispensing, touch the tip against the inside wall of the container and then depress the plunger to the second stop before releasing.

4. Ejecting the Tip:

  • Eject the used pipette tip into a designated waste container by using the tip ejector button or lever on the pipette.

Electronic or Motorized Pipettes:

1. Setting the Volume:

  • Turn on the electronic pipette and select the desired volume using the control panel or touchscreen interface.

2. Loading the Pipette:

  • Attach a disposable pipette tip to the electronic pipette.
  • Immerse the tip into the liquid sample.
  • Activate the aspiration function, and the pipette will automatically draw the liquid into the tip.

3. Dispensing the Liquid:

  • Position the pipette tip over the target container.
  • Activate the dispensing function, and the electronic pipette will dispense the liquid with precision.

4. Ejecting the Tip:

  • Some electronic pipettes have an automated tip ejection mechanism. Press the tip ejector button to release the used tip into a waste container.

General Tips for Pipetting:

  • Always use the appropriate-sized pipette tip for the selected volume range.
  • Ensure the pipette is properly calibrated and maintained according to the manufacturer’s guidelines.
  • Perform pipetting tasks on a level surface.
  • Use consistent and gentle pipetting techniques to avoid introducing errors.
  • Pipette in a slow and controlled manner to ensure accuracy.
  • For both manual and electronic pipettes, follow laboratory safety guidelines and wear appropriate personal protective equipment.

It’s important to note that specific pipetting techniques may vary slightly based on the design and features of the pipette, so always refer to the manufacturer’s instructions for the particular pipette model being used.