Pipetting Method of Pipette
Pipetting methods for pipettes are divided into two types, namely, forward pipetting and reverse pipetting.
1. Forward pipetting
This technology uses a blow-out function to ensure complete transfer of liquid. Press the pipette button to stop. Put the suction nozzle into the liquid surface 2-3mm, gently release the button to return to the origin. Carefully lift the applicator and dock on the container wall to remove excess liquid.
Press the button to the stop point and the liquid is discharged. Hold the button for a while and continue to press the button to the second stop point (ie, blow out). This step will empty the nozzle to ensure accurate liquid transfer. Release the button and return to the origin. If you need to change the nozzle, continue to pipette.
2. Reverse pipetting
Using this technique, more than the set range of liquid will be inhaled, and the pipetting does not require a blowout function so that excess liquid remains in the pipette.
This technique is recommended when transferring highly viscous liquids, bioactive liquids, easily foaming liquids or very small amounts of liquid. Press the pipette button to the second stop point, immerse the nozzle at 2-3mm below the liquid level, and gently release the button to return to the origin.
Carefully lift the applicator and dock on the container wall to remove excess liquid. Tap the button to the stop point, exit the set range, and continue to hold down the button, the liquid will remain in the pipette without being transferred. The excess liquid is discarded with the suction nozzle or returned to the original container.