Pipettes Calibration and Selections
Pipette is a graduated glass tube used to transfer a small-volume solution. As a ubiquitous tool in laboratories, pipettes come in many shapes and sizes. They are produced by many different manufacturers, but they perform the same functions, usually with specifications of 1ml, 2ml, 5ml, 10ml, and so on.
To ensure higher accuracy and better repeatability of the pipette ( such as Advanced Half Autoclave Single Channel Fixed Pipettes, Advanced Fully Autoclave Single-Channel Adjustable Pipettes, Electronic Control Digital Pipettes, Standard Single Channel Fixed Pipettes), it is very essential to periodically perform the maintenance procedure. The pipette should be regularly maintained based on the use frequency, but at least once every 3 months. It must be operated by trained personnel.
Types of pipettes
Incomplete efflux pipette, with the zero graduation above.
Complete efflux pipette, with zero graduation above or down.
Blow out type, with the fast flow velocity.
Pipettes calibration and selections
Pipette calibration is considered an essential part of the laboratory regime. It is used to transfer or dilute precise volumes of fluid. So it’s very important to understand accuracy and precision. After the cleaning procedure, the pipette needs to be calibrated. Its calibration must be performed at room temperature (25 +/- 2℃). Moreover, the air bubbles should be removed from the pipette head. And then the operator could select the scale that needs to calibrate. Remember to conduct all the next procedures under the international calibration standard.
Cleaning Instructions for pipette
The pipette could be cleaned by being separated into two parts. The outer wall of the pipette should be cleaned regularly with 95% alcohol or 60% isopropanol. Next, wipe it with distilled water and dry it naturally. Its internal cleaning requires disassembly of the pipette’s lower part, where it could be cleaned with soapy water, detergent, or 60% isopropanol at first.
And then rinse it with double distilled water and make it dry. Finally, the surface of the piston is better to be coated with a thin layer of lubricious silicone by using a cotton swab. For long-term maintenance, the internal part of the pipette needs to be better cleaned.
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