Pipette Use and Pipetting Method
Range adjustment
If you want to adjust the pipette from large volume to small volume, follow the normal adjustment method, and turn the knob counterclockwise; but if you want to adjust from small volume to large volume, you can first rotate the scale knob clockwise to over the scale of the range and then adjust it back to the set volume, which guarantees the highest accuracy of the measurement.
During this process, never push the button out of the range, or it will get stuck in the internal mechanism and damage the pipette.
Pipette tip installment
It is wrong to knock on the tip box a few times when putting the pipette tips on the pipette. By doing so, it will bring damage to the internal parts of the pipette (such as springs).
The correct way is to insert the pipette vertically into the tip and rotate it slightly to make it tightly bond. If it is a multi-channel (standard 8/12 Channels Adjustable Pipettes and Advanced Half Autoclave 8/12 Channels Adjustable Pipettes ) pipette, you can align the first line of the pipette with the first tip, then insert it obliquely, and shake it in the front-rear direction. The marking of the grip is slightly over the O-ring and the connecting part can be seen to form a clear seal.
Pipetting notes
Ensure the pipette, tip, and liquid are at the same temperature before pipetting. When pipetting the liquid, the pipette is held upright and the tip is inserted 2-3 mm below the surface. Before aspirating, you can first aspirate the liquid several times to wet the liquid suction nozzle (especially if the liquid is thick or has a different density from water).
Pipetting methods
Before pipetting, ensure that the pipette, tip, and liquid are at the same temperature. When pipetting the liquid, the pipette is held upright and the tip is inserted 2-3 mm below the surface. Before aspirating, you can first aspirate the liquid several times to wet the liquid suction nozzle (especially when you want to absorb a liquid that is thick or different in density from water). Two pipetting methods can be used at this time.
1. Forward pipetting
Press the button to the first stop with your thumb, then slowly release the button to return to the origin (suck a fixed volume of liquid). Then press the button to the first stop to discharge the liquid, and then stop for a while and continue to press the button to the second stop to blow out the residual liquid. Finally, release the button.
2. Reverse pipetting
It is generally used for transferring highly viscous liquid, biologically active liquid, easily foaming liquid, or very small amounts of liquid. Press the button first to the second stop and slowly release the button to the origin. Then press the button to the first stop point to discharge the liquid with the set range, hold the button at the first stop point (do not press down again), and remove the tip with residual liquid.
