Misuse of Pipettes
It is well known that pipetting cycles include attaching a tip, aspirating a liquid, dispensing a liquid, and removing a tip.
Modern pipettes are mostly gas piston pipettes. The principle of the pipette is that the piston moves through the telescopic movement of the spring to achieve aspiration and discharge.
Under the push of the piston, part of the air is exhausted, the liquid is sucked in by atmospheric pressure, and then the air is pushed out by the piston to discharge the liquid. When using the pipette, it is easy to control the speed and strength of pipetting when working with the spring’s stretchability. However, pipettes of this principle require a tight bond between the tip and the pipette, so the problem is coming!
In order to make the pipette tip connection stable, the user often needs to press the tip with a hand, and in the long run, it is easy to cause repeated hand damage. Not only that, because the force is too large, more force is needed when removing the tip. In the worst case, the tip must be manually removed, thereby increasing the risk of contamination.
In addition, in addition to the above-mentioned damage to the body, such an operation is also likely to cause wear of the tip of the pipette, making the combination of the tip and the tip of the gun more difficult! Different user’s exertion will have a negligible impact on the repeatability of the experiment!
At present, the ideal solution is to combine the pipette and the tip into a specific sealing system, which ensures the repeatability of each seal, reduces the artificial force applied to the tip, and finally achieves the best-pipetting accuracy and precision.