://Method of Pipetting

Method of Pipetting

Before pipetting, ensure that the pipette, tip, and liquid are at the same temperature. When pipetting the liquid, the pipette is held upright and the tip is inserted 2-3 mm below the surface. Before aspirating, you can first aspirate the liquid several times to wet the liquid suction nozzle (especially when you want to absorb a liquid that is thick or different in density from water). Two pipetting methods can be used at this time.
The first is the forward pipetting method. Press the button to the first stop point with your thumb, then slowly release the button to return to the origin (suck a fixed volume of liquid). Then press the button to the first stop to discharge the liquid, and then stop for a while and continue to press the button to the second stop to blow out the residual liquid. Finally, release the button.
The second is the reverse pipetting method. This method is generally used to transfer highly viscous liquids, biologically active liquids, easily foaming liquids or very small amounts of liquids. The principle is to inhale more liquid than the set range first, and it is not necessary to blow out the residual liquid when transferring the liquid. Press the button to the second stop point, slowly release the button to the origin, and after sucking up, lean against the wall of the container to return excess liquid along the wall to the container. Then press the button to the first stop point to discharge the liquid with the set range, continue to hold the button at the first stop point (do not press down again), remove the gun head with residual liquid, and discard it after sucking it up. Leaning against the wall of the container will allow excess liquid to flow back to the container along the wall.

2018-11-09T07:57:01+00:00September 20th, 2018|