://How to Use the Pipette?

How to Use the Pipette?

1. Capacity setting
For the adjustable pipette, it is usually operated in the range of 10% to 100%. The optimal pipetting capacity is in the range of 35% to 100%. At this time, it is less affected by the operation technique and can better protect the pipetting. Accuracy and precision. For example, to remove 600 uL of liquid, it is best to choose a 100 to 1000 uL adjustable pipette instead of 500 to 5000 uL.

2. Install the tip
Installation method: Insert the lower end of the pipette vertically into the tip, turn it left and right, and tighten it.

Be careful not to use excessive force, otherwise, the internal components will be loose, and even the knob of the adjustment scale will be stuck or the pipette sleeve will be bent.

3. Rinse the tip
When an organic solvent or a highly volatile liquid is removed, a volatile gas forms a negative pressure in the sleeve chamber, causing leakage. Rinse the pipette tip 4 to 6 times to make the gas in the sleeve room saturated, and the negative pressure disappears automatically, which can effectively prevent liquid leakage. However, do not rinse the tip when the sample temperature is very high or low.

4. Aspirate
There are two ways to aspirate: positive aspiration, reverse aspiration.
Positive aspiration refers to the normal method of aspiration. Press the button to the first step to release the solution and release the button. Press the first gear first, then press most of the liquid, then press the second gear to discharge the remaining liquid.

Reverse aspiration refers to pressing the button directly to the second gear and then releasing it so that more liquid will be inhaled. Just press the first gear when the liquid is discharged. The multi-inhalation liquid can compensate for the surface adsorption inside the tip. The reverse aspiration is generally used in combination with the pre-flushing and aspirating method and is suitable for viscous liquids, volatile liquids and small volume pipetting.

5. Draining
There are three ways of draining: draining along the inner wall, draining above the liquid level, and draining below the liquid level. The usual “draining along the inner wall” steps can be seen below:

(1) Stick the tip of the nozzle to the inner wall of the container and keep it inclined from 10° to 40°, and smoothly press the button to the first gear. After a slight stop, press the second gear to discharge the remaining liquid; when discharging dense or viscous liquid, stay The time is a little longer.
(2) Press the button and lift the pipette to lift the tip of the container out of the container wall.
(3) Release the button.

6. Remove the tip
The tip can be removed by pressing the tip ejector with a little force. If the tip is installed too tightly, it can be removed by hand. Discard the tip into a suitable waste collector.

2019-02-26T05:47:37+00:00February 26th, 2019|