How To Ensure Accuracy During The Use Of A Pipette
Replace the pipette tip method: the most accurate.
(1) Adjust the knob to the desired scale position, tips: First, it can be slightly more than some, then turn back to the scale. Be careful not to exceed the maximum scale to avoid human error.
(2) Insert tip: When aseptic operation, pay attention to the action is fast and correct, can not touch the tip by hand, the intensity of the action should be consistent, prohibiting waste action. For normal operation, use the thumb-index finger to tighten, but the hand can’t touch the tip of the pipette.
(3) Finger pressure to the first stage, pipette tip contact reagent. The white tip enters the liquid surface 1 mm, the yellow tip 2-3 mm, and the blue tip 2-4 mm to avoid errors caused by the influence of hydraulic pressure.
(4) Slowly aspirate the reagent, prohibit the speed too fast, and the liquid enters the tip.
(5) After removing the tip, first gently lean against the inner wall of the container to allow the liquid on the outer wall of the tip to flow down.
(6) Stick the tip on the inner wall of the target container, gently press it to the first section and press out the liquid.
(7) The tip slides slightly on the inner wall of the container, retaining a small amount of liquid at the tip, removing the target container, and removing the tip from the finger, taking care not to spill the reagent.