How to Choose a Pipette Tip?
The pipette tip is a consumable that is used to help avoid cross-contamination. In general, there is no problem with using a pipette tip for forward pipetting. However, some pipette tips are not necessarily suitable for some special situations. The design of the partial pipette tip does not fully consider the possibilities of various applications, and does not leave enough space in the tip. So when you need to use a liquid that needs to inhale extra capacity, such as a reverse pipetting, or when the liquid being removed is rich in protein and is prone to foaming, “tragedy” can happen at any time. You may find that the liquid is in intimate contact with the filter element after aspiration. As a result, pollution is still likely to spread. In order to control the risk of contamination caused by “intimate contact between liquid and filter element”, some brands have double filter elements in the tip or a closure mechanism (the filter element is automatically closed when the liquid contacts the filter element, so that the liquid cannot be discharged), but Still can’t change the fact that precious samples are lost. The best option at this point is to purchase a tip that leaves enough space in the tip to ensure that the liquid is protected from contact with the filter element even in special applications.
What is a low adsorption tip?
The accuracy and accuracy of pipetting not only requires matching of the tip and pipette, but also tips that are suitable for liquid properties. We often find a phenomenon in operation that when a standard tip (PP) is used to remove a liquid having a small surface tension (for example, a liquid containing a detergent), it is easy to leave a film on the inner surface of the tip. The reagents and samples used in many DNA and protein assays typically contain a detergent. Therefore, in the experiments of such applications, the case of more liquid residue is ubiquitous. Residues of the liquid can result in inaccuracies and inconsistencies in the pipetting results, as well as loss of some expensive samples. The development of low adsorption tips is a common problem in order to improve liquid residue. Different suppliers use different technologies to produce low-adsorption tips, so they differ in their consistency, hydrophobicity, and chemical resistance. There are two common production processes for low adsorption tips: physical polishing and chemical coating. The former utilizes a polishing technique to treat the surface of the tip, making the surface of the tip very smooth to reduce the residue of the liquid, and more reliably ensuring the safety of the sample. However, the mold during polishing will age during the production process, and the quality of the low adsorption tip cannot be guaranteed. The chemical coating method adds a layer of hydrophobic agent to the surface of the tip, which may present a risk of dissolution and introduce contamination. The safest and most reliable is the low adsorption tip produced by Sartorius using patented technology to help reduce liquid residue and increase sample recovery!
Pipette Tip selection universal standard
1. Look at the material: Select the inert material of the tip to ensure chemical compatibility and avoid dissolution. At present, the most commonly used is pure PP material. The quality of the supplier’s materials has a standard that is easy to judge. Just look at the color of the tip to know. “Colored tips can help you quickly identify its range” – is this really the case? In fact, the more important purpose is to cover the impure material. Impurities in the tip made of recycled plastic are very easy to be perceived by the naked eye, and when added, the impurities are “invisible”, so transparent tips are usually better-looking tips.
2. Look at the appearance: a good quality tip will have a high appearance. They have a smooth surface, no burrs, a flat cut, a uniform shape and no straight bends, so as to ensure that the liquid does not easily remain and obtain a precise pipetting effect.
3. Look at the certificate: If you purchase a nozzle without a heat source, the supplier should provide the certificate for the batch of the product.