Factors Affecting the Accuracy of Pipettes
A pipette is an indispensable tool for the experimental operation. For the top master, it is important that the experimental error is the smallest and the repeatability is good. Then pay attention to the correct pipette.
Factors affecting the precision of the pipette
(1) Temperature: When the room temperature is low, the hand temperature is high, which causes the air to expand, and the error often occurs when inhaling the cold solution.
(2) Airtightness: the joint between the pipette tip and the pipette body, and the long-term wear between the cylinders inside the gun causing errors
(3) Suction speed: it is easy to cause bubbles in the tip, and it will pollute the gun head.
(4) Volatilization of reagent: When a reagent having high volatility is absorbed, steam enters the tip of the pipette, and the internal pressure is increased. As a result, when the liquid is pressed out, the pressure becomes large, and an error occurs.
In addition, Hawach does not recommend the use of ordinary pipettes for strong volatile liquid transfer. If it is necessary to remove highly volatile liquids, the pipette should be disassembled immediately after the pipetting to allow the vapor to evaporate. At the same time, it is recommended to use external devices. Piston pipette.
Please note that the aspiration rate and the discharge rate should not be too fast, and should be slowly slowed down. Quickly sucking the pipette for 1mL and above, it is easy to cause liquid overshoot. Excessive draining increases the amount of liquid remaining.