Different Methods Used to Remove Liquids in Different Situations

The first situation is for transferring regular liquids. Press the button to the first stop point. Put the dripper of the pipette below the liquid surface and then release the button slowly. Until the dripper of the pipette suctions the liquids, remove the dripper from the liquid surface and slant it on the wall of the reagent bottle to flow out the excess liquid.

Move the pipette to the sampling position and gently press the button to the first stop point. And then continue pressing the button down to the second stop point in order to drain out the liquid in the emitter.

The second situation is transferring the thick liquid and the foaming liquid. When operating with a small amount of liquid and changing the dripper repeatedly, press the button down to the second stop point, place the dripper of the pipette below the reagent surface, and then slowly release the button to suction the liquid.

After the dripper is filled with liquid, the dripper is withdrawn from the liquid surface and slanted on the wall of the reagent bottle to flow out the excess liquid. Move the pipette to the sampling position, and continue pressing the button down to the first stop point to drain out the preset liquid.

Removing liquids from a pipette in various situations can be accomplished using different methods, depending on the specific requirements and the type of pipette being used. Here are different methods used to remove liquids from pipettes in different situations:

  1. Blowout:
    • This method involves expelling the remaining liquid in the pipette by blowing gently into the pipette tip. It is commonly used with manual pipettes for general laboratory applications.
  2. Reverse Pipetting:
    • Reverse pipetting is a technique where the pipette is set to dispense a volume slightly larger than needed, and then the excess liquid is drawn back into the pipette tip. This method is used when high precision and accuracy are required, as it reduces the risk of liquid carryover.
  3. Positive Displacement Pipettes:
    • Positive displacement pipettes use a disposable piston that comes into direct contact with the liquid. This design eliminates the risk of contamination and is particularly useful for viscous or volatile liquids.
  4. Micropipette Ejector Button:
    • Some micropipettes have an ejector button on the pipette body or tip cone that can be pressed to expel the liquid from the pipette tip.
  5. Disposable Tips:
    • Many pipettes are used with disposable tips, which can be easily removed and discarded after dispensing liquids. This minimizes the risk of cross-contamination and eliminates the need for liquid removal.
  6. Aspiration System:
    • In more advanced laboratory settings, automated pipetting systems with aspiration and dispense modules can be used. These systems can precisely control liquid removal and dispensing, making them suitable for high-throughput applications.
  7. Pipette Filler Bulbs:
    • Bulb pipette fillers, also known as pipette bulbs or pipette fillers, are rubber or silicone bulbs that can be attached to the top of a pipette to create a vacuum for aspiration and dispensing.
  8. Vacuum Aspiration Systems:
    • Vacuum aspiration systems are used in situations where precise control of liquid removal is critical, such as in cell culture work. These systems provide adjustable vacuum pressure for efficient aspiration.
  9. Serological Pipettes:
    • Serological pipettes are designed with a large opening at the top and are often used in liquid transfer from containers. They typically rely on gravity to drain the liquid.
  10. Multichannel Pipettes:
    • Multichannel pipettes, commonly used in high-throughput applications, have multiple channels for simultaneous pipetting. Liquid removal in these pipettes is similar to single-channel pipettes but on a larger scale.
  11. Electronic Pipettes:
    • Electronic or motorized pipettes offer precise control over liquid removal and dispensing through electronic mechanisms. These pipettes are used in research and clinical laboratories for high-precision applications.
  12. Peristaltic Pumps:
    • Peristaltic pumps can be used in situations where accurate and continuous liquid removal is required. They use a rotating roller to create a pumping action, making them suitable for a wide range of volumes and viscosities.

The choice of method for removing liquids from pipettes depends on factors such as the type of pipette, the volume being dispensed, the precision required, and the specific application in the laboratory or industry. Different pipetting methods offer varying levels of control and accuracy to meet the needs of diverse applications.