Common Explanations for Pipettes

A pipette, also known as a pipette, is a measuring tool that transfers liquid from the original container to another container within a certain range. It is widely used in biology, chemistry, and other fields.

Pipettes are commonly used in laboratories for the precise measurement and transfer of liquids. Here are some common explanations or reasons for using pipettes:

  1. Precision and Accuracy:
    • Pipettes are designed to deliver precise and accurate volumes of liquids. They allow researchers and scientists to dispense specific amounts of reagents, samples, or solutions with high precision, crucial for obtaining reliable experimental results.
  2. Quantitative Analysis:
    • Pipettes are essential tools for quantitative analysis in various scientific disciplines. They enable the accurate measurement of volumes needed for experiments, assays, and analytical procedures.
  3. Reproducibility:
    • The use of pipettes ensures reproducibility in experiments by allowing researchers to handle and dispense liquids consistently. This is critical for obtaining reliable and comparable data across multiple trials.
  4. Sample Preparation:
    • Pipettes are commonly used in sample preparation for assays, reactions, and other laboratory procedures. Accurate pipetting is crucial when preparing standards, controls, and samples for analysis.
  5. Reducing Cross-Contamination:
    • Disposable pipette tips are often used to minimize the risk of cross-contamination between samples. Changing tips for each sample prevents the transfer of residues from one sample to another, ensuring the integrity of the results.
  6. Handling Small Volumes:
    • Micropipettes are designed for handling small volumes of liquids, often in the microliter or nanoliter range. This capability is particularly important in fields such as molecular biology, where small volumes of reagents are used in reactions.
  7. Cell Culture:
    • Pipettes are commonly used in cell culture work for the accurate dispensing of media, buffers, and other solutions. This is critical to maintain the proper conditions for cell growth and experiments.
  8. Drug Discovery and Clinical Research:
    • In pharmaceutical research and clinical laboratories, pipettes play a crucial role in drug discovery, diagnostics, and various analytical procedures. Accurate dispensing is essential for drug formulation and testing.
  9. PCR (Polymerase Chain Reaction):
    • In molecular biology, pipettes are used in PCR and other DNA amplification techniques. Accurate pipetting is vital for preparing reaction mixtures with precise concentrations of DNA, primers, and other components.
  10. Enzyme Assays and Biochemical Studies:
    • Pipettes are employed in enzyme assays, protein quantification, and various biochemical studies where the accurate measurement of volumes is essential for the success of the experiment.
  11. Maintaining Sterility:
    • In microbiology and clinical laboratories, pipettes are used under sterile conditions to handle and transfer liquids without introducing contaminants. This is crucial for maintaining the integrity of cultures and diagnostic samples.

Understanding the principles of pipetting and using pipettes properly contribute to the reliability and validity of scientific experiments and analyses. Researchers must follow best practices for pipetting to ensure the accuracy and reproducibility of their work.

Standard Single-Channel Fixed Pipettes
Electronic Control Digital Pipette Epipette

The basic structure

Hawach pipette(Advanced Fully Autoclave Single-Channel Fixed Pipettes, Advanced Half Autoclave Single Channel Fixed Pipettes) is widely used in the clinical laboratory because of its simple structure and easy to use. Its basic structure mainly has the display window, the capacity adjustment part, the piston, the 0- shape ring, the suction tube and the suction head (suction nozzle) and so on several parts.

The working principle

The design basis of the micropipette commonly used in the clinic is Hooke’s law: that is, the long force of spring extension is proportional to the elasticity within a certain limit, that is, the volume of liquid in the pipette is proportional to the spring elasticity in the pipette.
There are two physical principles of micropipette loading: the use of air pad loading and the use of positive displacement with no air pad. The micropipette of these two different principles has different specific applications.

Common classification

According to the working principle, it can be divided into air displacement pipette and forward displacement pipette.
It can be divided into the single-channel pipette and multi-channel pipette according to the number of pipettes that can be installed simultaneously.

According to whether the scale can be adjusted, it can be divided into the fixed pipette and adjustable pipette.
According to the adjusting scale, it can be divided into the manual pipette and an electric pipette.
According to the special use can be divided into full disinfection pipette, large capacity pipette, bottle mouth pipette, continuous injection pipette, etc.

Clinical application

Clinical diagnostic laboratories, biotechnology laboratories, pharmaceutical and chemical laboratories, environmental laboratories, food laboratories.

Move the technique

Forward displacement method: Press the shift lever to the first stop point, then slowly release the button to return to the origin; Then discharge the liquid by pressing the pipette lever to the first stop position, and continue to discharge the residual liquid by pressing the pipette lever to the second stop position after a short pause. Finally, slowly release the pipette lever.

Reverse transfer method: First press the button to the second stop point, then slowly loosen the pipette lever to return to the origin. When discharging the liquid, push the pipette lever to the first stop point to discharge the liquid with the volume set. Continue to hold the pipette lever at the first stop point to remove the suction head with residual liquid and discard it.