Cleaning, Disinfection, and Pipetting Skills of Bottle-Top Dispenser
The selection of the appropriate suction head has a great influence on the accuracy of the final dispenser, so it is best to select the suction head used in the initial calibration of the dispenser, that is, the suction head recommended by the manufacturer. In operation, when the lower end of the sleeve handle of the dispenser enters the suction head, the dispenser can be swayed from side to side for 1 to 2 seconds and gently pressed down; if it is a bulk suction head, gently pressing the suction head in the direction of the dispenser with hand, then turning the suction head slightly for 1 to 2 seconds.
Range Control of Bottle-top Dispenser
Generally speaking, the range of consumption of dispensers is 10 ≤ 100% of the maximum range of liquid shifters. For specific liquid shifters, the accuracy of the liquid shifters decreases with the decrease of the liquid shifters. In general, the best range is 30 ≤ 100% of the maximum range of liquid shifters. In the process of adjusting the range, the principle of from large to small is obeyed in order to eliminate the mechanical error of the liquid shifters themselves.
Skills for Volatile Samples and High Viscosity Samples
It is necessary to rinse the liquid two times before moving the liquid, drain the liquid as soon as possible after the liquid absorption is completed, and reduce the loss of liquid volatilization as far as possible. In order to avoid damage to the piston, it is recommended to use a filter core suction head. A high-viscosity sample is necessary to move the liquid with the reverse discharge mode, and a residence time of 3 to 5 seconds is required for both suction and drainage.
Skills for Fine and Micro Samples and High Contagious Samples
It is necessary to move liquid with a reverse liquid transfer model. In order to reduce sample loss, a low-absorption suction head is recommended. For highly contagious samples, cross pollution in the liquid transfer process such as contamination of bottle-top dispenser tube can be greatly avoided by using a filter core suction head.