Adjustment of Pipettes Range

Pipettes are often used to remove a small amount of liquid in the laboratory. Micropipette first appeared in 1956, invented by Schnitger who is a scientist of the Institute of physiology and chemistry, Germany.

Pipettes of different specifications are equipped with different sizes of pipette tips. Shapes of different manufacturers are slightly different, but the working principle and operation method are basically the same.

Pipettes are a kind of precision instrument, when use and storage, should be careful to prevent damage and avoid affecting its range.

Adjustment of pipette range

Adjusting the range of a pipette is a crucial step to ensure accurate and precise volume delivery. Here are the general steps to adjust the range of a pipette:

  1. Select the Correct Pipette:
    • Ensure you have the correct pipette size for your desired volume range. Each pipette is designed for a specific volume range (e.g., 10-100 µL, 100-1000 µL, etc.).
  2. Set the Volume:
    • Turn the volume adjustment dial or knob to the desired volume. This can usually be found on the top or side of the pipette.
  3. Check Calibration:
    • Use a calibrated pipette or balance to verify the accuracy of the adjusted volume. Dispense the liquid onto a tared container or weighing boat and record the weight.
  4. Fine Adjustment (if available):
    • Some pipettes have a fine adjustment option that allows for minor tweaks in the volume setting. Use this feature if needed to achieve the exact volume required.
  5. Test with Water:
    • For a practical check, use distilled water to pipette the adjusted volume. Dispense it into a weighing boat placed on a balance, and confirm the weight matches the expected value.
  6. If the Volume is Incorrect:
    • If the dispensed volume is not accurate, re-adjust the pipette using the adjustment knob or dial. Repeat the calibration and testing steps until the desired volume is achieved.
  7. Record Adjustments:
    • Keep a record of the adjustments made, especially if you need to switch between different volumes frequently.
  8. Regular Maintenance and Calibration:
    • Periodically, send your pipettes for calibration and maintenance to ensure they continue to deliver accurate volumes.
  9. Adjustment Guidelines:
    • Follow the manufacturer’s guidelines and recommendations for adjusting the range of specific pipette models. Each pipette may have unique features and adjustment mechanisms.
  10. Use Suitable Tips:
    • Ensure you’re using tips that are compatible with the adjusted volume range. Using tips with an incorrect size can lead to inaccurate pipetting.
  11. Practice Good Pipetting Technique:
    • Regardless of the adjusted range, always use proper pipetting technique to ensure accurate and reproducible results.

Remember, precision and accuracy in pipetting are critical for obtaining reliable experimental data. If you encounter difficulties or are unsure about the adjustment process, consult the manufacturer’s instructions or seek guidance from a qualified expert.

When you adjust the range, if you want to adjust the volume from high to low , Press the normal adjustment method and turn the knob clockwise. But if you want to turn the volume from low to high, you can turn the dial counterclockwise, put the dial out of range, and then turn it back to the set volume, and the highest accuracy of measurement can be guaranteed. In this process, do not turn the button out of the range, or it will block the internal mechanical device, damage the pipette.

After using it, pipettes can be hung vertically on the straw rack, but be careful not to fall off.