The Correct Use of the Pipette

The researchers often use a pipette to absorb a small amount of reagents or solutions in the laboratory. The micropipette can be used frequently in the steps of dilution, coating, etc. The correct operation can ensure that the experiment is not wrong or the error is small, so the correct use of the micropipette is crucial.

1. hold a pipette

Hold the handle with the palm and four fingers, the index finger is close to the front hook structure, and the thumb is placed on the control button.

2. the selection range

After pulls the knob that adjusts the range, turn the knob to the appropriate range, and finally press the knob back to the original position. As shown in the figure, the maximum range of the pipette is 1000 microliters.

3. attach the pipette tips

Align the pipette perpendicular to the opening of the tip so that the pipette and the tips are firmly fixed together to ensure that there are no gaps, or the reagent will leak out.

4. Aspiration liquid

Press the button of the pipette to the middle position with your thumb, stick the tip into the liquid in half. Do not press the knob to the end. Otherwise, the volume of the liquid aspirated is not correct. Then gently release the thumb and the liquid is Aspirated.

Before aspirating, you can aspirate the liquid several times to wet the tips (especially when you want to absorb a liquid that is thick or different in density from water)

5. Dispense liquid

A. Advance pipetting method. Press the button to the first stop point with your thumb, then slowly release the button to return to the original point. Then press the button to the first stop to discharge the liquid, pause for a while and continue to press the button to the second stop to discharge residual liquid. Finally release the button.

B. Reverse pipetting. This method is generally used to transfer highly viscous liquids, biologically active liquids, and easily foaming liquids, or a very small amount of liquid. The principle is to first inhale more liquid than the set range, when transferring liquid, it is not necessary to blow out the residual liquid. Press the button first to the second stop and slowly release the button to the origin, then press the button to the first stop point to discharge the liquid with the set range, and keep the hold button at the first stop.

Point (don’t press down again).

6. Place the centrifuge tube

Place on the centrifuge tubes on the racks after well covered, preferably placed from left to right in order to avoid confusion when picking and placing.

7. Remove the tips

Press the white button on the side of the front end of the pipette tip, the tip will automatically fall off.

8. Recall the maximum range

Return the pipette to the maximum range to reduce damage to the spring inside the pipette. Be careful not to over twist when rotating the knob of the range, which will also cause damage to the spring.