Pipette Error Operation and Processing Method
As the most commonly used manual adjustable precision pipette in liquid operation, some wrong operations during the use will bring errors to the operation itself, and some will cause damage to the machine. Here is a brief summary:
ERROR: When assembling the tip, use the pipette to repeatedly hit the tip.
Correct: insert the tip, turn left and right to rotate the tightening tip
Analysis: The impact will cause the looseness of the internal gear combination of the pipette, and long-term operation will affect the accuracy of the pipette.
Wrong: the tip does not match the pipette, affecting the correct airtightness: use a quality-assured tip that matches the pipette
Analysis: The mismatch of the tips is mainly reflected in the impact on the airtightness. It will directly affect the accuracy of the pipette, and usually, the pipetting operation will be less than the set amount.
Error: Pipette tilting aspiration when aspirating
Correct: vertical pipetting
Analysis: The tilted state causes a change in the air pressure surface. It will affect the accuracy of pipetting.
Error: When the tip contains unprinted liquid, the pipette is placed flat on the table
Correct: hang the pipette vertically on the pipette holder
Analysis: The liquid in the tip contains unfilled liquid. After the pipette is laid flat, the liquid may flow into the pipette body, which will affect the internal components of the pipette, causing serious damage, poor pollution, etc. happened.
Error: Pipetting a small volume of liquid with a large pipette
Correct: The pipetting volume needs to be guaranteed to meet the inaccuracy and inaccuracy requirements within the range provided by the pipette.
Analysis: Because of the presence of air cushions, it is the main cause of pipette inaccuracy. The choice of pipette should be the maximum range, the closest to the selection principle of the liquid transfer amount of the target.
Error: draw a liquid with strong volatility
Correct: It is not recommended to use a common pipette for strong volatile liquid transfer
Analysis: If a highly volatile liquid must be removed, the pipette should be disassembled immediately after the pipetting to allow the vapor to evaporate. At the same time, an external piston pipette is recommended.
Error: Aspirate speed and discharge speed too fast
Correct: slow suction slow release
Analysis: Fast suction for liquid pipettes of 1mL and above, it is easy to cause liquid overshoot. Excessive draining increases the amount of liquid remaining.