Common Explanations For Pipettes
A pipette, also known as a pipette gun, is a measuring tool that transfers liquid from the original container to another container within a certain range. It is widely used in biology, chemistry, and other fields.
The basic structure
Hawach pipette is widely used in the clinical laboratory because of its simple structure and easy to use. Its basic structure mainly has the display window, the capacity adjustment part, the piston, the 0- shape ring, the suction tube and the suction head (suction nozzle) and so on several parts.
The working principle of
The design basis of the micropipette commonly used in the clinic is Hooke’s law: that is, the long force of spring extension is proportional to the elasticity within a certain limit, that is, the volume of liquid in the pipette is proportional to the spring elasticity in the pipette.
There are two physical principles of micropipette loading: the use of air pad loading and the use of positive displacement with no air pad. The micropipette of these two different principles has different specific applications.
According to the working principle, it can be divided into air displacement pipette and forward displacement pipette.
It can be divided into the single-channel pipette and multi-channel pipette according to the number of pipettes that can be installed simultaneously.
According to whether the scale can be adjusted, it can be divided into the fixed pipette and adjustable pipette.
According to the adjusting scale, it can be divided into the manual pipette and an electric pipette.
According to the special use can be divided into full disinfection pipette, large capacity pipette, bottle mouth pipette, continuous injection pipette, etc.
Clinical application of
Clinical diagnostic laboratories, biotechnology laboratories, pharmaceutical and chemical laboratories, environmental laboratories, food laboratories.
Move the technique
Forward displacement method：Press the shift lever to the first stop point, then slowly release the button to return to the origin; Then discharge the liquid by pressing the pipette lever to the first stop position, and continue to discharge the residual liquid by pressing the pipette lever to the second stop position after a short pause. Finally, slowly release the pipette lever.
Reverse transfer method：First press the button to the second stop point, then slowly loosen the pipette lever to return to the origin. When discharging the liquid, push the pipette lever to the first stop point to discharge the liquid with the volume set. Continue to hold the pipette lever at the first stop point to remove the suction head with residual liquid and discard it.