Calibrating Multichannel Pipettor To Prevent Errors
Widely used in chemistry, molecular biology research, and medical tests in the lab as well, a pipettor is a handy instrument for transporting a measured volume of liquid. It can draw up and dispense liquid by creating a vacuum above the liquid-holding chamber and releasing this vacuum. From single piece glass pipettors to complex adjustable or electronic pipettor, Hawach provides pipettors in several designs for various purposes, and in different levels of accuracy and precision too.
On one hand, a pipettor needs are accurate to the degree with the delivered volume being equal to the specified volume, on the other hand, for precision, it needs to be concerned with the closeness of several measurements to each other. For example, a pipettor might be consistently inaccurate but this inaccuracy could be very precise you still can get the reproducible result out of your samples.
The scientists always prefer the perfect pipetting with accuracy and precision for their applications. It is important to work hard to get maxim accuracy and precision and reduce the level of uncertainty at the same time. To reduce the risk of error in manual liquid handling, you need to choose Hawach pipetting system, consisting of the highest quality pipettors professionally maintained and calibrated. And they can be used with the high quality matched tips. With Hawach pipetting products on hands, the correct pipetting angle, speed, and a smooth, consistent pipetting rhythm will help you get better results.
In order to prevent errors when using a multi-channel pipettor, you must learn how to calibrate the main characteristics of the multichannel pipettor:
1. Large-sized central pipetting control button, independent tip removal button.
2. Ergonomically designed finger rest that fits the hand and is easy to hold.
3. Real one-handed operation-regardless of the left or right hand, you can easily set the pipetting volume with one hand.
4. Range lock to prevent unintentional range changes.
Calibration steps for multichannel pipettors
a. Adjust the pipettor to the volume to be calibrated and select the appropriate tip;
b.Adjust the balance;
c. Suck and blow distilled water back and forth 3 times to make the tip wet, wipe the tip with gauze;
d. Hold the pipettor vertically, immerse the tip into the liquid surface 2 ~ 3mm, and slowly (1 ~ 3 seconds) suck the distilled water consistently;
e. Remove the tip from the liquid surface and lean against the tube wall to remove the liquid outside the tip;
f.Put the pipettor into the weighing beaker at an angle of 30 °C. Slowly and uniformly press the pipettor to the first position, wait for 1 to 3 seconds, and then press it to the second position to completely discharge the liquid in the tip;
g. Record the weighing value;
h. Dry the outside of the tip;
i. Weigh 10 times according to the above steps;
j.Take the average value of 10 measurements as the weight of distilled water absorbed by the final pipettor, and calculate the volume according to the Z factor of distilled water listed in Appendix 1;
k. Adjust the pipettor according to the calibration result.
The above is all about sharing how to calibrate in order to prevent mistakes when using multi-channel pipettors today. I hope it will be helpful for everyone to use this device in the future.